magnetic cell based
Quantum sensors for biomedical applications | Nature Reviews …
NV-based magnetic microscopy was also used to detect cancer biomarkers 63 (Fig. 4a,b). In this study, SKBR3 cancer cells were labelled with HER2-specific MNPs (where HER2 is human epidermal growth ...
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Magnetic responsive cell-based strategies for …
Abstract. The potential of magnetically assisted strategies within the remit of cell-based therapies is increasing, creating new opportunities for biomedical platforms …
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The Design and Control of Magnetized Cell-Based Microrobot for
Magnetized cell-based microrobot motion under in Helmholtz's uniform magnetic field. A-D, represent the location of magnetic cell-based microrobot at different time point (0 s, 1 s, 4 s and 6 s ...
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Magnetic-Based Human Tissue 3D Cell Culture: A Systematic …
Recent magnetic-based 3D (m3D) cell culture systems can be used for this purpose. These systems are based on exposing magnetized cells to magnetic fields by levitation, bioprinting, or ring formation to promote cell aggregation into 3D structures. However, the successful development of these structures is dependent on several …
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Immunomagnetic separation is a suitable method for …
Magnetic-activated cell sorting (MACS) techniques provide an alternative to fluorescence-activated cell sorting (FACS), however, the potential impact of MACS-related beads on the biophysical and pharmacological properties of the ion channels were not studied yet. ... A conventional flow cytometry-based cell sorting using positive selection …
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An Overview of Single-Cell Isolation Techniques
These target cells can be isolated with methods such as fluorescence-activated cell sorting (FACS), magnetic-activated cell sorting (MACS), microfluidics, and laser capture microdissection (LCM) ... PAN has a comparatively high yield of cells based upon the efficiency and specificity of the antibody. PAN is expensive, however, as a …
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Magnetic Cell Separation | Cell Isolation Technology
Magnetic cell separation, also known as immunomagnetic cell separation or magnetic cell sorting, involves targeting cells for selection or depletion using antibodies or ligands directed against specific cell surface antigens.
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Magnetic bead-based adsorption strategy for exosome isolation
The magnetic bead-mediated selective adsorption strategy (MagExo), which tends to adsorb EVs on the surface of magnetic beads selectively, can separate EVs from plasma and cell culture media (CCM) with high purity, resulting in two times higher yield than EVs obtained by ultracentrifugation (Figure 1; Fang et al., 2021). However, in a ...
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Magnetic particle separation, a short review
Commercial magnetic particle separation. One of the earliest works in magnetic capturing using bulk magnets was reported by Miltenyi in [1]. In this work, a Magnetic Cell Sorter (MACS) from Miltenyi Biotec is used to separate cells labeled with magnetic particles from non-labeled cells. Three basic steps can be observed: the objects of interest ...
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Magnetically actuated microrobots as a platform for stem cell
Magnetic microrobots were developed for three-dimensional culture and the precise delivery of stem cells in vitro, ex vivo, and in vivo. Hippocampal neural stem cells attached to the microrobots proliferated and differentiated into astrocytes, oligodendrocytes, and neurons. Moreover, microrobots were used to transport colorectal carcinoma ...
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Frontiers | Magnetic Force-Based Microfluidic …
Live cell manipulation is an important biotechnological tool for cellular and tissue level bioengineering applications due to its capacity for guiding cells for separation, isolation, concentration, and patterning. Magnetic force …
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Microfluidics for cell separation | SpringerLink
Cells attached to magnetic beads follow the direction of the parallel magnetic lines, while other cells follow the direction of the liquid. Using a similar technique, recently Pamme and Wilhelm introduced continuous magnetic cell sorting based on free-flow magnetophoresis (Fig. 2A) . The separation relies on the variation in the uptake of ...
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Basic Principles and Recent Advances in Magnetic …
Figure 9. Label-free magnetic cell sorting. ( A) Microfluidic cell separation based on the difference in magnetic repulsive forces between two cells of different size in a paramagnetic salt solution. The separation efficiency …
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Recent Advances on Magnetic Sensitive Hydrogels in Tissue Engineering
Abstract. Tissue engineering is a promising strategy for the repair and regeneration of damaged tissues or organs. Biomaterials are one of the most important components in tissue engineering. Recently, magnetic hydrogels, which are fabricated using iron oxide-based particles and different types of hydrogel matrices, are becoming …
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Magnetically driven microfluidics for isolation of circulating tumor cells
When a magnetic field perpendicular to the direction of flow is applied by a permanent magnet adjacent to the nickel microstructure, cells in a paramagnetic solution are deflected laterally because of magnetic repulsion force. 119 C, Mechanism underlying the isolation of particles with different sizes based on water sheath flow under a local ...
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Magnetic separations
Whereas very simple magnetic devices, such as MagLev-based systems, have been used for the analysis of cells to detect diseases (Ashkarran & Mahmoudi, 2021; Ge et al., 2020), these systems are based on the suspension of diamagnetic cells/particles in paramagnetic liquids and generally used to measure density. However, other devices …
ادامه مطلبAcoustic and Magnetic Stimuli-Based Three-Dimensional Cell …
In a magnetic-based 3D cell culture platform, cells are aggregated by changing the magnetic force. Magnetic fields can manipulate cells through two different methods: positive magnetophoresis and negative magnetophoresis. Positive magnetophoresis is a way of imparting magnetic properties to cells by labeling them …
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Considerations for high-yield, high-throughput cell enrichment
In fact, this has been demonstrated by clinical trials using magnetic bead-based, FDA-approved CliniMACS and ISOLEX 300i CD34 + cell isolation systems for treatment of leukemia and other ...
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Magnetic bioprinting of stem cell-based tissues
An attractive approach in cell therapies and medically oriented nanotechnologies is to interface magnetic nanoparticles with cells. This will endow the cells with sufficient magnetization for theranostic applications and for external magnetic field manipulation.. In tissue engineering, one challenge is to produce tissue analogues …
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Dual-neodymium magnet-based microfluidic separation device
The magnet-mediated separation of the microparticles or cells in microfluidic devices has recently been developed by following two approaches: separating magnetic microparticles from non-magnetic ...
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Microfluidic Devices for Magnetic Separation of Biological Particles…
Abstract. Separation of microparticles and cells serves a critical step in many applications such as in biological analyses, food production, chemical processing, and medical diagnostics. Sorting on the microscale exhibits certain advantages in comparison with that on the macroscale as it requires minuscule sample or reagents volume and thus …
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Magnetic cell separation
The column-based MACS is a favored technique for separation of cells due to the high magnetic field gradient. The column-based MACS includes a column that is packed with randomly entangled magnetically susceptible wires [68] or ferromagnetic spheres [69] and it is surrounded by an electromagnet, Fig. 7.1A.After activating the …
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Publisher's Note: "Magnetic force-based cell …
This corrects the article "Magnetic force-based cell manipulation for in vitro tissue engineering" in volume 7, ... license; 94 (c) Levitational self-assembly of cell …
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Endoscopy-assisted magnetic navigation of biohybrid soft
However, vision-based control of microrobots, to deep and narrow spaces inside the body, remains a challenge. Here, we report a soft and resilient magnetic cell microrobot with high biocompatibility that can interface with the human body and adapt to the complex surroundings while navigating inside the body.
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Microtechnology-based methods for organoid models
Magnetic levitation method. Magnetic levitation (ML, Fig. 2e) is another method for organoid production 48, 49. In this method, cells are cultured and incubated with bacteriophages, polydisperse ...
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Magnetic cell separation | Miltenyi Biotec | Deutschland
Immunomagnetic cell separation is based on antibodies coupled to magnetic beads. During incubation with a cell suspension, the antibody/bead complex binds to cells expressing the corresponding epitope. When the cell suspension is placed into a magnetic field, magnetically labeled cells are retained, while unlabeled cells can be removed. To …
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Magnetic bioprinting of stem cell-based tissues
An attractive approach in cell therapies and medically oriented nanotechnologies is to interface magnetic nanoparticles with cells. This will endow the …
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Magnetic Force-Based Microfluidic Techniques for Cellular and …
Magnetic force-based cell sheet engineering eliminates the need for thermosensitive surfaces that are used in conventional methods to form cell sheets. It was reported that magnetic force-based manipulation of cells could be used to construct and harvest keratinocyte sheets (Ito et al., 2004a). MCL-labeled human keratinocytes were …
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Hybrid magnetic and deformability based isolation of circulating …
The capture efficiency can reach up to 90% at 3ml/h coated with immune beads and at 1.5 ml/h without beads. (n=3) (B) All MCF-7 cells were coated with magnet beads in bright field. Bead binding is an important step in the magnetic-based isolation. Scale bar: 50 µm. (C) An MCF-7 cell was surrounded by immune magnetic beads. …
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Complex-shaped magnetic 3D cell-based structures for tissue engineering
By providing magnetic cells as building blocks, this work proposes the fabrication of mechanically strong and complex-shaped 3D-like tissues using magnetic-force based tissue engineering (Mag-TE) in a simple, cost-effective and time-saving fashion. A plethora of organized tissues could be created using such universal approach that …
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